Development of harmful algal bloom monitoring methods and forecast system for sustainable aquaculture and coastal fisheries in Chile (Monitoring of algae in Chile)


SATREPS MACH English Poster is available!

STREPS-MACH Download .pdf SATREPS MACH English Poster (for the general public) In the middle of January 2021, our SATREPS MACH team has updated the project’s poster in English available for all public.

MACH research assistant has entered to FONDECYT Postdoctoral program

    On early January 2021, we have received good news from one of our research assistants. Dr. Ignacio Rilling who works for our project at Universidad de La Frontera has been accepted as postdoctoral program fellow in FONDECYT.     Dr. Rilling has been working with MACH project since November 2018. He mainly works for metabarcoding analysis of samples collected by the relevant institutions.    During his postdoctoral program he will focus on dynamics of antibiotic resistance genes in the soil environment. We would be happy if the experience he gained from the project will be useful in the future. Comment from Dr. Rilling: “I am deeply honored and grateful to […]

Five unit of “Suitcase Lab” have arrived to Chile for rapid and on-site detection of harmful algae in Chilean coastal waters

   During January of 2021, 5 units of “Suitcase Lab” previously introduced in our web sites (, finally have arrived to Chile from Japan to boost our research and outreach activities.    The Suitcase Lab, as we call it, consists of an all-in-one portable field tool that allows extract DNA from a sample of seawater containing microalgae. The extracted DNA will be used to amplify DNA fragments that are unique and distinctive to the species, so the algal species in the water sample can be identified with tools inside the suitcase lab.     Currently, the 5 units are still in an experimental and validation phase at La Frontera University, given […]

We held 2nd UFRO genome informatics course “Introduction to Transcriptomic Analysis 2021”

    On January 2021, we held the 2nd summer course of genomic informatics entitled “Introduction to Transcriptomic Analysis” at University of La Frontera (UFRO) as a training course for experimental biological students and researchers. Course contents covered topics of genome informatics ranging from basic to practical issues. We developed a program that combines lectures, demonstrations, exercises and hands-on session and final presentation so that participants can effectively acquire knowledge and skills.      Professor Andrés Ávila (UFRO), Dr. Mikihiko Kawai (Kyoto University), and Giovanni Larama (UFRO) were in charge of the program, and gave lectures in corporation with invited speakers, Professor Fumito Maruyama (Hiroshima University) and Dr. Enrique Ostria-Gallardo […]

SATREPS MACH Brochures is updated!

   At the beginning of January 2021 our SATREPS MACH team has updated project  brochure in English available for all public.     SATREPS MACH English Brochures (for general public)  

SATREPS MACH Brochures is ready for distribution!

  At the beginning of December 2020 our SATREPS MACH team has prepared project brochures in English and Spanish (two version) available for all public. SATREPS MACH English Brochures SATREPS MACH Spanish Brochures (for general public) SATREPS MACH Spanish Brochures (for researches etc)

Dr. Nagai, National Research Institute of Fisheries Science, visits Hiroshima University to perform “one-day DNA sequencing”

  On 3-6th November 2020, as a part of the SATREPS MACH project, Dr. Nagai from the National Research Institute of Fisheries Science visited the Environmental Genomics and Ecology Laboratory of Hiroshima University.   The purpose of his visit was to quickly detect possible algicidal bacteria and utilize the information for algae bloom regression. To do so, we aimed to examine bloom samples for bacterial DNA amplification, purification, and DNA sequencing of sample bacteria by a NGS (next generation sequencer) within 15 hours.   In this experiment, the time required for the whole process to test 18 samples was 10 hours, successfully achieving the 15 hours goal. The time can […]

MACH project publishes a scientific paper on “Suitcase Lab” for rapid and on-site detection of specific harmful algae in Chilean coastal waters

  In November 2020, Chilean and Japanese researchers in the MACH project published a research paper entitled “Suitcase Lab: New portable and deployable equipment for rapid detection of specific harmful algae in Chilean coastal waters” in Environmental Science and Pollution Research.   This paper introduces the process of water sampling from the field, DNA extraction, and LAMP (loop-mediated isothermal amplification) to detect the target harmful algae (Alexandrium catenella as a model species which is a major problem in Chile) was performed in 2 hours.   Detection of harmful algae species using a suitcase lab was previously introduced on our website ( We are greatful to announce that the results were […]

MACH project releases a protocol on “Metagenomic Sequencing Library Preparation”

  On October 23th, 2020, Chilean and Japanese researchers associated with MACH project released a research protocol entitled “16S and 18S metabarcoding analysis for Chilean coastal waters harmful algal blooms” on the website:   This protocol describes the metagenome section of our other publication (Int J Environ Res Public Health, 2020, 17(20). doi:10.3390/ijerph17207642) in much more details so that the operation of the section is repeated correctly. The protocol was established based on the hands-on metabarcoding analysis through our current monitoring program.   This protocol is released as “Open Access” and can be obtained from the following site. Please click here for details.    

MACH project members publish the first article: protocol on monitoring Harmful Algal Blooms in Chile

On October 20th, 2020, Chilean and Japanese researchers associated with the MACH project published a research article entitled “Protocols for Monitoring Harmful Algal Blooms for Sustainable Aquaculture and Coastal Fisheries in Chile” in the International Journal of Environmental Research and Public Health. This article represents the project’s first milestone: the standardization of Harmful Algal Bloom (HAB) monitoring methodologies from several perspectives, including physical, chemical, microbiological, and molecular biological water sample analyses. All the project members diligently contributed to establishing the protocols and performing field and laboratory studies with compliance with the protocol to deliver the results. The article focuses on Chilean HAB monitoring; however, this step-wise standardized protocol is beneficial […]

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